The goal of this study is to assess improvement of hair d. and hair beauty parameters after 3 mo supplementation with a food supplement in healthy Japanese female subjects. The food supplement contains L-cystine, pantothenic acid, biotin and millet dry aqueous extract, ingredients known to improve hair growth and hair appearance. Healthy Japanese female subjects, with non-illness related, light diffuse hair loss and thinning of hair, were enrolled in the Single center open-label study. Investigational product (IP) was taken four tabs/day from Visit 3 (Day 1) to End of Study (EoS) Visit (approx. 90 days of supplementation) to assess hair growth co-efficiency and hair d. [total number of hair counts on the test area (per cm2)] with photo-trichogram at Day 90 compared to Day 1 Baseline. Hair quality and beauty parameters (volume, shine, softness, strength, smoothness, thickness, dryness, vitality) were assessed by means of a 10-points-scale by hairstylist at Day 1 Baseline and Day 90 to compare. The 118 subjects were finally included in the statistical anal. and it was found that overall hair d. was significantly increased from 146.82/cm2 to 152.42/cm2 after 3 mo supplementation. Anagen phase hair d. was increased from 137.35/cm2 to 142.81/cm2 also with significant difference. Hair growth co-efficiency (A/T) however showed no significant level change. For the hair appearance and beauty assessment, all parameters were improved significantly after 3 mo supplementation. The study results suggest that intake of the food supplement which contains L-cystine, pantothenic acid, biotin and millet dry aqueous extract is effective for improvement of hair d. and also the overall hair beauty in Japanese women.