Objective To investigate the correlation of 25-hydroxy vitamin D [25(OH)D] levels with triglyceride (TG), total cholesterol (TC), high d. lipoprotein cholesterol (HDL-C), low d. lipoprotein cholesterol (LDL-C), homocysteine (Hcy), glycosylated Hb A1c (HbA1c) and glycosylated albumin (GA) in healthy pregnant women. Methods A total of 204 healthy pregnant women in Chancheng Central Hospital of Foshan City from July to Oct. 2019 were selected as the research subjects. The levels of 25(OH)D, TG, TC, HDL-C, LDL-C, Hcy, HbA1c and GA were detected in venous blood samples from all subjects and compared. The correlation between serum 25(OH)D and other indexes were analyzed. Results The difference of HbA1c among different age was statistically significant (P<0.05). There was statistical significance in Hcy level among all groups (P<0.05). Serum 25(OH)D and Hcy (r=-0.211, P=0.003), TG (r=-0.157, P=0.025) showed a neg. correlation. Conclusion It is important to observe the changes of blood lipid level, take a reasonable diet, adopt a healthy lifestyle, and serum 25(OH)D level for the diagnosis and prediction of cardiovascular disease in healthy pregnant women.